High Abundance Proteins: Proteomer’s Thorns in the Flesh?
نویسندگان
چکیده
منابع مشابه
High Abundance Proteins: Proteomer’s Thorns in the Flesh?
Identification of low-abundance proteins is one of the major challenges in Proteomics because of the high dynamic range of the protein concentration in the biological samples [1]. Therefore, one of the prime objectives of the proteomers is to reduce the dynamic protein concentration range to shed a light on the “low-abundance proteome” or sometimes referred as “Hidden Proteome” [2]. The develop...
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Depletion of high-abundance proteins is regarded as a critical sample preparation step for most plasma proteomic analyses and profiling strategies. This report describes a process that rapidly and reproducibly precipitates high-abundance disulfide-rich proteins, including albumin and transferrin, from serum and plasma. A low volume of concentrated reducing agent, viz. dithiothreitol (DTT) or tr...
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ژورنال
عنوان ژورنال: Journal of Proteomics & Bioinformatics
سال: 2017
ISSN: 0974-276X
DOI: 10.4172/jpb.1000e35